Regulatory
Strong
Part:BBa_K320002:Design
Designed by: Perrudet Christian Group: iGEM10_EPF_Lausanne (2010-10-18)
Strong promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 62
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was artificially made from two primers containing the E. coli consensus sequences for promoter and RBS with an appropriate spacing.
References
- [http://mic.sgmjournals.org/cgi/reprint/132/2/565.pdf Activity of two strong promoters cloned into Bacillus subtilis]